Botox is used medically to treat certain muscular conditions, and cosmetically to remove wrinkles by temporarily paralyzing muscles. It is made from a neurotoxin called botulinum toxin that is produced by the bacterium Clostridium botulinum. ... Botulinum toxin is used to reduce fine lines and wrinkles. it is also used in myofacial pain and headaches caused by muscle tension and TMD.